Amino Acid Column Hplc

Pre column derivatizing reagents most typically used for amino acid analysis are o phthalaldehyde phenyl isothiocyanate pitc fluorescamine and dansyl chloride.

Amino acid column hplc.

In an amino column you have an nh2 group at the end of the carbon chain attached to the silica. Lc ms analysis of amino acids without derivatization. Therefore pre column derivatization can be considered appropriate when intending to analyze a somewhat limited variety of samples with high sensitivity. The use of internal and external standards is crucial.

It uses ultra high purity fully porous spherical silica as a matrix to provide a good peak for amino acid analysis using a unique bonding process and a more thorough tailing process. We are planning to develop method on analysis of amino acids in food matrix cereals pulses oil seeds etc by hplc with pre or post column derivatization methods since this is new to us so could. However pre column derivatization has gained importance and can be achieved by a broader range of derivatization reagents. Whether you are looking to separate proteins peptides or amino acids the intrada series of columns has a solution for you.

It is based on ultra high purity and all hole spherical silica gel. Nh2 hplc columns description. Full chromatographic resolution of isobaric compounds like leu and ile. Experimental conditions aaa on production 1200sl using eclipse plus c18 2 1 or 4 6 x 50mm 1 8u.

Luna 5 µm c18 2 100 å lc column 250 x 4 6 mm ea part. The characteristics of the column will be affected by the chemical properties of this functional group. Optimized stationary phase for the separation of amino acids and other amine containing compounds. The nh2 hplc column uses the innovative filler bonding technology unique to hawach.

Description of amino acid hplc columns. The nh2 group is a group where n has a lone e. Since most of the amino acids have no absorption in the ultraviolet. Post column derivatization was typical.

Hydrolysed samples amino acids are derivatized for sensitive detection separated by hplc.